*Click test name for more information including; Specimen Requirements, Storage and Transport, Specimen Rejection Criteria, Turn around Time and CPT codes.

Test Name Methodology Test Description Specimen Requirements Storage and Transport Specimen Rejection Criteria Turn Around Time (TAT) CPT Codes*
TdT Flow Cytometry

Staining for terminal deoxynucleotidyl transferase (TdT) highlights normal precursor B-cells in bone marrow, tonsils and lymph nodes and normal thymocytes. TdT is expressed by the blasts in most cases of B and T-lymphoblastic lymphoma/leukemia, 10-15% of cases of acute myeloid leukemias, some mixed lineage leukemias and some non-hematopoietic neoplasms (Merkel cell carcinoma).

Testing for this antigen is typically performed as a part of the acute leukemia add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

T-cell receptor gamma/delta Flow Cytometry

Staining for TCR gamma/delta highlights a minor subset (<5%) of normal mature CD3+ T-cells in bone marrow and peripheral blood, while representing the majority of T-lymphocytes in mucosal tissues. It is also expressed by certain subtypes of mature T-cell non-Hodgkin lymphoma (e.g. hepatosplenic gamma/delta T-cell lymphoma, primary cutaneous gamma/delta T-cell lymphomas) and subsets of post-thymic T-cell lymphoma/leukemia.

Testing for this antigen is typically performed as a part of the T-cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

T-cell receptor alpha/beta Flow Cytometry

Staining for TCR alpha/beta highlights the majority (>95%) of normal mature CD3+ T-cells in bone marrow and peripheral blood samples. TCR alpha/beta is also expressed on many subtypes of mature T-cell non-Hodgkin lymphomas and in a small subset of post-thymic T-cell lymphoblastic lymphoma/leukemia.

Testing for this antigen is typically performed as a part of the T-cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

MYELOPEROXIDASE Flow Cytometry

Staining for cytoplasmic myeloperoxidase (MPO) highlights normal myeloid cells of neutrophil lineage at all stages of maturation (blasts to mature granulocytes), as well as subsets of monocytes. It is also expressed in the blasts of acute myeloid leukemias and some mixed lineage acute leukemias.

Testing for this antigen is typically performed as a part of the acute leukemia add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

LAMBDA Flow Cytometry

Staining for immunoglobulin lambda light chain highlights subsets of mature B-cells (by surface staining) and plasma cells (by intracytoplasmic staining), as well as the neoplastic cells in mature B-cell lymphomas and plasma cell neoplasms. The kappa:lambda ratio is used to assess these neoplasms for clonality.

Testing for this surface antigen is typically performed as a part of the leukemia/lymphoma screening panel, while testing for cytoplasmic lambda is part of the plasma cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

KAPPA Flow Cytometry

Staining for immunoglobulin kappa light chain highlights subsets of mature B-cells (by surface staining) and plasma cells (by intracytoplasmic staining), as well as the neoplastic cells in mature B-cell lymphomas and plasma cell neoplasms. The kappa:lambda ratio is used to assess these neoplasms for clonality.

Testing for this surface antigen is typically performed as a part of the leukemia/lymphoma screening panel, while testing for cytoplasmic kappa is part of the plasma cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

HLA-DR Flow Cytometry

Staining for HLA-DR highlights normal mature B-cells, activated T-cells, monocytes/macrophages, dendritic cells and CD34+ blasts. Additionally, it is expressed on the leukemic cells of acute myeloid leukemias (with the notable exception of acute promyelocytic leukemia), lymphoblastic and mixed lineage leukemia, and by neoplastic lymphoid cells in mature B-cell and T-cell lymphomas.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

FLAER Flow Cytometry

FLAER (fluorescein-labeled proaerolysin) is a bacteria-derived prototoxin which binds directly to the glycophosphatidylinositol (GPI) anchor in the membrane of leukocytes. It is used in the flow cytometric assessment for paroxysmal nocturnal hemoglobinuria (PNH), as a direct measurement of GPI expression on granulocytes and monocytes.

Testing for this antigen is typically performed as a part of the PNH analysis. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

FMC7 Flow Cytometry

Staining for FMC7 highlights a subset of mature B-lymphocytes, which represent >50% of peripheral blood B-lymphocytes in adults. FMC7 antibodies actually highlight an epitope present on the extracellular domain of the CD20 molecule, which is detected variably at different stages of B-cell maturation due to sensitivity of the specific antibodies to the variable levels of membrane cholesterol characteristic for these stages of maturation. FMC7 is detected on many mature B-cell lymphomas/leukemias and is characteristically absent in CLL/SLL with classic immunophenotype, which also shows decreased expression of CD20.

Testing for this antigen is typically performed as a part of the B-cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD235A Flow Cytometry

Staining for CD235a (Glycophorin A) detects erythroid precursors and erythrocytes and is also expressed by the neoplastic blasts in rare acute erythroblastic leukemias. In our laboratory it is used for delineating the erythrocytes in the analysis for paroxysmal nocturnal hemoglobinuria.

Testing for this antigen is typically performed as a part of the PNH analysis. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD138 Flow Cytometry

Staining for CD138 highlights precursor B-cells (hematogones) and mature plasma cells. It is also typically expressed in plasma cell neoplasms (MGUS, myeloma).

Testing for this antigen is typically performed as a part of the plasma cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD117 Flow Cytometry

Staining for CD117 highlights CD34+ stem cells, myeloblasts, promyelocytes, erythroblasts, some monoblasts, megakaryoblasts and mast cells. CD117 is also expressed by the blasts in acute myeloid leukemias, some subsets of T-acute lymphoblastic leukemias (early T-precursor ALL), some mixed lineage acute leukemias, and by mast cells in systemic mastocytosis.

Testing for this antigen is typically performed as a part of the acute myeloid leukemia add-on panel and the systemic mastocytosis add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD103 Flow Cytometry

Staining for CD103 may highlight subsets of activated T-cells, monocytes and mast cells. It is also aberrantly expressed by neoplastic B-cells in hairy cell leukemia and splenic B-cell lymphoma/leukemias.

Testing for this antigen is typically performed as a part of the B-cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD81 Flow Cytometry

Staining for CD81 highlights leukocytes and normal plasma cells. It is most often used in the diagnostic work-up of plasma cell neoplasms, where complete or partial loss of CD81 expression is a common finding.

Testing for this antigen is typically performed as a part of the plasma cell neoplasm ad-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD79b Flow Cytometry

Staining for CD79b detects normal B-cells at all stages of maturation associated with expression of surface immunoglobulin. Staining for this antigen is used in panels of antibodies used to characterize B-cell neoplasms, as it is often lost as a part of the aberrant B-cell phenotypes seen in this context (e.g. it is often negative in classic CLL/SLL).

Testing for this antigen is typically performed as a part of the B-cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD64 Flow Cytometry

Staining for CD64 detects subsets of normal mature granulocytes and monocytes at all stages of maturation. Increased expression of CD64 on neutrophils correlates with sepsis. CD64 expression is also present in most acute myeloid leukemias and some precursor B lymphoblastic leukemias.

Testing for this antigen is typically performed as a part of acute myeloid leukemia add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD59 Flow Cytometry

Staining for CD59 detects hematopoietic cells of all lineages. CD59 is a GPI anchor-linked protein, and it is most often used in the diagnostic work up for paroxysmal nocturnal hemoglobinuria (PNH), where loss of this CD59 expression is used to identify abnormal PNH clones in erythrocyte, monocyte and granulocyte populations.

Testing for this antigen is typically performed as a part of the PNH analysis. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD57 Flow Cytometry

Staining for CD57 detects a subset of CD8+ T-cells, T-large granular lymphocytes. It is used more often in the detection and quantitation of the latter cells in normal samples and T-large granular lymphocyte proliferations. CD57 may also be expressed by precursor T- and some precursor B-lymphoblastic lymphoma/leukemia cases.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD56 Flow Cytometry

Staining for CD56 detects normal NK cells and subsets of CD8+ cytotoxic T-cells. Partial, weak CD56 expression may also be detected in subsets of normal plasma cells and on immature myeloid and monocytic cells in regenerative (reactive) processes. CD56 expression is present on a large variety of hematopoietic neoplasms, including T-cell lymphomas, B- and T-lymphoblastic leukemia/lymphoma, NK and T-large granular lymphocyte proliferations, acute myeloid leukemias and plasma cell neoplasms (MGUS, myeloma).

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD45 Flow Cytometry

Staining for CD45 detects most cells of hematopoietic origin at all stages of maturation. The combination of CD45 intensity and side-scatter is used in flow cytometry for acurate separation of the main leukocyte populations in bone marrow and peripheral blood samples, and for detection of abnormal cell populations. CD45 expression is regarded as a highly useful marker of hematopoietic lineage in staining panels for poorly differentiated neoplasms. However, some hematopoietic neoplasms may be negative for CD45. These include myelomas, some plasmablastic lymphomas, some acute myeloid and lymphoblastic leukemia cases, and some cases of T-anaplastic large cell lymphoma.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD38 Flow Cytometry

Staining for CD38 detects normal plasma cells, activated T-cells and B-cells, precursor B-cells (hematogones) and progenitor cells (blasts). Most plasma cell neoplasms are strongly positive for CD38. Most acute myeloid and lymphoblastic leukemias are also positive for this antigen. CD38 expression in chronic lymphocytic lymphoma is highly correlated with unmutated IGVH genes (“unmutated CLL”) and an unfavorable prognosis.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel and the plasma cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD34 Flow Cytometry

Staining for CD34 detects progenitor cells, including stem cells, myeloid blasts and early lymphoid progenitors (“stage I hematogones”). It is also expressed by the blasts of most acute myeloid leukemias and precursor B lymphoblastic lymphoma/leukemia, and by 80-85% of cases of precursor T-lymphoblastic lymphoma/leukemia. Acute monoblastic leukemias lack CD34 expression in a large proportion of cases.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD33 Flow Cytometry

Staining for CD33 detects normal monocytes and some normal immature myeloid cells. It is often expressed by myeloblasts and monoblasts in acute myeloid leukemias, and by the blasts of some precursor B lymphoblastic lymphoma/leukemia cases and of rare cases of precursor T-lymphoma/leukemia. It is also expressed in a large subset of T-anaplastic large cell lymphomas.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD27 Flow Cytometry

Staining for CD27 detects some normal mature B-cell subsets (germinal center and memory B-cells), plasma cells, T-cells and NK cells. It is most often used in the diagnostic work-up of plasma cell neoplasms, where loss of CD27 expression is a common finding.

Testing for this antigen is typically performed as a part of the plasma cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD25 Flow Cytometry

Staining for CD25 (TAC, IL-2 receptor alpha chain) detects activated B-cells, T-cells and monocytes. CD25 is strongly expressed by hairy cell leukemia cells and variable expression may also be present in other B-cell lymphomas and T-cell lymphomas (most characteristically HTLV-related adult T-cell lymphoma/leukemia). Aberrant expression of CD25 is seen in on mast cells in systemic mastocytosis.

Testing for this antigen is typically performed as a part of the B-cell neoplasm add-on panel and the systemic mastocytosis add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD24 Flow Cytometry

Staining for CD24 detects mature granulocytes and normal mature and immature B-cells at all stages of maturation. It is also expressed in most mature B-cell and B-lymphoblastic neoplasm. In our laboratory this marker is used for defining (gating on) the mature granulocytes to assess for antigen loss as a part of the PNH analysis.

Testing for this antigen is typically performed as a part of the PNH analysis. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD23 Flow Cytometry

Staining for CD23 detects subsets of peripheral blood mature B-lymphocytes and some B-cell found in the mantle zone of the B-lymphoid follicles and normal follicular dendritic cells. It is expressed by some B-cell neoplasms (e.g. chronic lymphocytic leukemia, hairy cell leukemia, marginal zone lymphoma).

Testing for this antigen is typically performed as a part of the B-cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD22 Flow Cytometry

Staining for surface CD22 detects normal mature and immature B-cells and normal basophils. It is also expressed at variable intensity by most mature B-cell non-Hodgkin lymphoma and precursor B-lymphoblastic lymphoma/leukemia, as well as in some mixed lineage acute leukemias (B-lymphoid/myeloid), which may show mostly cytoplasmic expression of this antigen.

Testing for this antigen is typically performed as a part of the B-cell neoplasm add-on panel. Cytoplasmic staining for CD22 is also performed as a part of acute leukemia add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD20 Flow Cytometry

Staining for CD20 detects mature B-cells and precursor B-cells at some stages of maturation. It is also expressed at variable intensity in most mature B-cell non-Hodgkin lymphomas, many cases of precursor B lymphoblastic lymphoma/leukemia (typically weak and variable in the latter), and some myelomas [characteristically myeloma with t(11;14)]. While regarded as a highly specific B-lineage associated marker, it has been rarely found in some mature T-cell lymphomas and carcinomas. Notably, any of these B-cell neoplasms may become CD20-negative following therapy with agents targeting CD20 (e.g. Rituximab).

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD19 Flow Cytometry

Staining for CD19 detects normal mature and immature B-cells at all stages of maturation, and most normal plasma cells. CD19 is also expressed in most mature B-cell non-Hodgkin lymphomas, precursor B-cell lymphoblastic lymphoma/leukemia and by the myeloblasts of some acute myeloid leukemias [most characteristically, AML with t(8;21)]. The loss of CD19 expression is a part of the abnormal immunophenotype of a large proportion of plasma cell neoplasms (including MGUS and myeloma).

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD16 Flow Cytometry

Staining for CD16 detects mature granulocytes, myeloid precursors, subsets of activated monocytes, NK cells and subsets of CD8+ T-cells. CD16 expression is also present in abnormal NK cell proliferations including NK cell leukemias. CD16 is a GPI-anchored protein. The complete or partial loss of CD16 expression on mature granulocytes is characteristic for paroxysmal nocturnal hemoglobinuria (PNH).

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD15 Flow Cytometry

Staining for CD15 detects mature granulocytes and immature myeloid cells (excluding CD34+ blasts), as well as mature and immature monocytes. It may also be expressed by the leukemic blasts of some acute myeloid leukemia (AML). As co-expression of CD15 and CD34 is abnormal, this combination of markers may be used for minimal disease detection in AML.

Testing for this antigen is typically performed as a part of the acute myeloid leukemia add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD14 Flow Cytometry

Staining for CD14 is used mainly to detect normal mature monocytes. It is also expressed at low levels by a subset of granulocytes and immature myeloid cells. Additionally, it may be expressed at variable levels on leukemic monocytes in some acute myeloid leukemias with monocytic differentiation.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD13 Flow Cytometry

Staining for CD13 detects mature granulocytes, myeloid precursors at all stages of maturation, mature and immature monocytes. It is also expressed by acute myeloid leukemias, a large proportion of precursor B-cell acute lymphoblastic leukemias, and a significant proportion of anaplastic large cell lymphomas.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD11c Flow Cytometry

Staining for CD11c detects mature granulocytes, myeloid precursors (excluding CD34+ blasts) and mature and immature monocytes. CD11c is also expressed by acute myeloid leukemias, some precursor B-lymphoblastic leukemias, some T-anaplastic large cell lymphomas, and some mature B-cell neoplasms (hairy cell leukemia, hairy cell leukemia variant, splenic B-cell lymphoma, marginal zone lymphoma.

Testing for this antigen is typically performed as a part of the B-cell neoplasm add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD11b Flow Cytometry

Staining for CD11b detects mature granulocytes, myeloid precursors (excluding CD34+ blasts) and monocytes. Abnormal CD11b expression may be seen on myeloid blasts of some acute myeloid leukemias, on lymphoid blasts of precursor B lymphoblastic leukemias and precursor T-lymphoblastic leukemias, and in a large proportion of T- anaplastic large cell lymphomas. As co-expression of CD11b and CD34 is abnormal, this combination of markers may be used for minimal disease detection in AML.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD10 Flow Cytometry

Staining for CD10 highlights normal precursor B-cells (“hematogones”), mature B-cells found in benign germinal centers and mature granulocytes. It is also expressed at variable intensity in B-cell neoplasms including follicular lymphoma, Burkitt lymphoma, diffuse large B-cell lymphoma, and precursor B-cell lymphoblastic lymphoma/leukemia. It is also expressed by the neoplastic T-cells in angioimmunoblastic T-cell lymphoma.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD8 Flow Cytometry

Staining for CD8 detects a normal T-cell subset (cytotoxic). It is also expressed at normal or abnormal levels by some mature T-cell non-Hodgkin lymphomas, T-large granular lymphocyte leukemias, and many T-lymphoblastic lymphoma/leukemias (where it is often co-expressed with CD4).

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD7 Flow Cytometry

Staining for CD7 detects normal T-lymphocytes, NK cells and thymocytes. It is also expressed at normal or abnormal levels by most mature T-cell non-Hodgkin lymphomas and precursor T-cell lymphoblastic lymphomas/leukemias.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD5 Flow Cytometry

Staining for CD5 detects normal T-lymphocytes. It is also expressed at normal or abnormal levels by most mature T-cell non-Hodgkin lymphomas and precursor T-cell lymphoblastic lymphomas/leukemias. Dim CD5 expression may be found on some normal B-cell subsets. Additionally, CD5 expression is characteristically present in some subsets of B-cell non-Hodgkin lymphoma, including chronic lymphoid leukemia/small lymphocytic lymphoma, mantle cell lymphoma and less commonly, some marginal zone lymphomas and lymphoplasmacytic lymphomas.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD4 Flow Cytometry

Staining for CD4 detects a normal T cell subset (helper/inducer) and is also expressed at lower levels by normal mature and immature monocytes, and by myeloid precursors at the promyelocyte/myelocyte stage. It is also present on normal thymocytes where it may be co-expressed with CD8. The antibody is also expressed on some mature T-cell lymphomas (e.g. anaplastic large cell lymphomas, mycosis fungoides and some peripheral T-cell lymphomas,NOS) and in many of T-lymphoblastic lymphoma/leukemias (where it is often co-expressed with CD8).

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD3 Flow Cytometry

Staining for surface CD3 detects normal T-lymphocytes and is an ideal marker of normal mature T-cell enumeration in blood and marrow samples. Surface CD3 is also expressed at normal or abnormal levels by most mature T-cell non-Hodgkin lymphomas and some precursor T-cell lymphoblastic lymphomas/leukemias. Cytoplasmic CD3 expression is present in all T-lymphoblastic lymphomas/leukemis and in some mixed lineage (T-lymphoid/myeloid) acute leukemias.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel. Cytoplasmic CD3 is also performed as a part of the acute leukemia add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

CD2 Flow Cytometry

Staining for CD2 detects normal T-lymphocytes and NK cells. It is also expressed at normal or abnormal levels by most mature T-cell non-Hodgkin lymphomas and precursor T-cell lymphoblastic lymphomas/leukemias. Abnormal CD2 expression also occurs on monocytes in some acute myeloid leukemias and on mast cells in systemic mastocytosis.

Testing for this antigen is typically performed as a part of the leukemia/lymphoma screening panel and the systemic mastocytosis add-on panel. For customized testing panels including this antigen, please contact the Medical Director of the Flow Cytometry laboratory.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184, 88185, 88187, 88188, 88189

High Sensitivity PNH Evaluation   Flow Cytometry

Markers are CD14, CD15, CD24, CD45, CD59, CD64, CD235a (Glycophorin A), and FLAER. The sensitivity of this assay is 0.01% for granulocyte and monocyte PNH clones and 0.01-0.1% for RBC PNH clones. This assay is consistent with the most recent International Clinical Cytometry Society (ICCS) Guidelines.

•Peripheral blood: 5 ml (1-2 mL minimum) EDTA or sodium heparin tube. Please provide recent CBC report. Bone marrow specimens are not acceptable.

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184(x1), 88185(x7), 88187(x1)

Standard Leukemia/Lymphoma Panel – 23 markers   Flow Cytometry

Markers are CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD13, CD14, CD16, CD19, CD20, CD33, CD34, CD38, CD45, CD56, CD57, 7AAD, HLA-DR, surface kappa, and surface lambda. Additional markers may be added if initial screen is abnormal.

"•Bone marrow aspirate or peripheral blood: 5 ml (1-2 ml minimum), EDTA or sodium heparin tube. Please provide recent CBC report. •Bone marrow core: 1-2 cm minimum core length in RPMI.
•Fresh, unfixed tissue: Submit specimen in RPMI.
•Fluids and FNAs: Equal parts RPMI and specimen.
"

Maintain specimen at room temperature. Do not refrigerate or freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

Click here to reference the specimen rejection criteria.

24 hours

88184(x1), 88185(x22), 88189(x1). Additional 88185 CPTs may be added if initial screen is abnormal.

Methodology Test Name Test Description Specimen Requirements Storage and Transport Specimen Rejection Criteria Turn Around Time (TAT) CPT Codes*

*The CPT codes provided with our Test Descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.