Test Name: AB/PAS/H
Methodology: Special Stains
Alcian Blue/PAS/Hematoxylin Stain Kit is intended to identify acidic and neutral mucins in tissue sections on the Artisan™ Link and Artisan™ Link Pro Staining Systems. This procedure clearly separates the acidic and neutral mucins by color and can be used to distinguish all mucins in tissue sections. Alcian Blue pH 2.5 stains the acid mucin blue while the Schiff’s reagent stains the neutral mucins pink to red. Mixtures of the two mucins will appear purple due to the positive reactions with both Alcian Blue and Schiff’s reagent. A hematoxylin counterstain is then applied to impart a blue/black color to the nuclei.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen type or one (1) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and two to three (2-3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered.
Storage and Transport
Use cold pack during transport of paraffin blocks and unstained slides. Cold pack should not be placed in direct contact with the specimen during shipping
Specimen Rejection Criteria
Turn Around Time (TAT): 2-3 Days
CPT Codes*: 88313
*The CPT codes provided with our Test Descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.