Test Name: JAK2 Exon 12-14 Mutation Analysis  

Methodology: Molecular Genetics

Test Description

RT-PCR and bi-directional sequencing to detect non-V617F mutations in exons 12-14 and most of exon 15, corresponding to the majority of the JAK2 pseudokinase domain. Exon deletion mutations are detectable. Testing is performed on plasma for increased sensitivity whenever possible. V617F analysis is recommended before or concurrently with this test. Exon 12-14 Mutation Analysis may be ordered separately, with concurrent V617F testing, by reflex after negative V617F testing, or as part of the MPN Reflex Panel.

Specimen Requirements

•Peripheral blood: 4 mL in EDTA tube.
•Bone marrow: 2 mL in EDTA tube.

Storage and Transport

Use cold pack for transport, making sure cold pack is not in direct contact with specimen. Ship same day as drawn whenever possible; specimens <72 hours old preferred.

Specimen Rejection Criteria

Click here to reference the specimen rejection criteria.

Turn Around Time (TAT): 5 Days

CPT Codes*: JAK2 Exon 12-14 Only: 81403 JAK2 V617F and EXON 12-14 run concurrently or by reflex:  81270, 81403

*The CPT codes provided with our Test Descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.