Test Name: Targeted Solid Tumor Sequencing Panel
Methodology: Molecular Genetics
The Targeted Solid Tumor Sequencing Panel utilizes the Illumina TruSight Tumor 15 (TST15) assay which is a targeted cancer gene panel dedicated to detecting variants by covering the implicated coding regions of 15 genes associated with solid tumors. TST15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of the most commonly mutated genes in 7 cancer types (Lung, Melanoma, Breast, Colon, Ovarian, Gastric, and Prostate). It detects low-frequency variants down to a 5% minor allele frequency (MAF) and requires only 20 ng of DNA isolated from FFPE tumor tissue. TST15 allows for parallel sequencing of multiple cancer-related genes without the need to perform several sequential tests.
FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Storage and Transport
Use cold pack during transport of paraffin blocks and unstained slides. Cold pack should not be placed in direct contact with the specimen during shipping.
Specimen Rejection Criteria
Turn Around Time (TAT): 24 hours
CPT Codes*: 81445
*The CPT codes provided with our Test Descriptions are based on AMA guidelines and are for informational purposes only. Correct CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed.